Webb9 apr. 2007 · Hoechst 33342 was used to elucidate which cell states of the cell cycle might have captured PI maximally. The data for M. frederiksbergense are presented in Figure 3 . A clear assignment was difficult to make due to dye pumping (Fig. 3 A, 48 h, gate 1) and quenching of the Hoechst 33342 fluorescence intensities when high amounts of PI (Fig. … Webbis compromised, PI can enter and stain the DNA of dead cells, thus providing a marker for cell death. With the addition of Hoechst 33342, a specific DNA fluorochrome, we can …

Hoechst 33342/PI Double Staining Kit Biorbyt

WebbHoechst 33342 Page 1 of 2 PSF006 Hoechst 33342 Catalog number Product MW Size 40046 Hoechst 33342, 10 mg/mL in H 2 O 561.93 10 mL 40047 Hoechst 33342, trihydrochloride trihydrate 615.98 100 mg Storage and Handling Store Hoechst 33342, 10 mg/mL in H 2 O at 4°C, protected from light. Store Hoechst 33342, trihydrochloride … http://www.cyto.purdue.edu/cdroms/cyto3/8/data/icrf/hpi.htm calvin barry thunder bay

Hoechst 33342/PI Double Staining Kit Biorbyt

Webb2 nov. 2024 · CCK-8, ELISA, western blot, and Hoechst 33342/PI double staining were performed to detect the pyroptosis of BV-2 cells. Conditioned medium (CM) from BV-2 cells exposed to different treatments was used to investigate its effect on neuronal injury. WebbProduct Name: Hoechst 33342, 20mM in H2O; 2’-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5’-bi-1H-benzimidazole Manufacturer/Supplier: AnaSpec, Inc. www.anaspec.com 34801 Campus Drive Fremont, CA 94555 Tel: 510-791-9560 Fax: 510-791-9572 Email: [email protected] Catalog Number AS-83218 Unit Size 5mL 2. … WebbIn 1978, another DNA-binding fluorochrome, Hoechst 33342, ... (Hoechst – PI count) normalized to the 2 h time point, and e) the percentages of PI-positive dead cells with … calvin barry law firm