Hoechst concentration
Nettet25. sep. 2024 · Hoechst typifies a chemical biology probe with remarkable characteristics, possessing good permeability, high affinity (K d ∼1–10 nM) and specificity, and a fluorescent turn-on of ∼30- to 40-fold upon DNA binding with a large 100 nm Stokes shift, 16 when they bind to adenine-rich regions in the minor groove of DNA (Figure 1a). 17 … Nettet31. mar. 2024 · Hoechst uptake by live cells is an active process and should be performed under optimal culture conditions depending on cell type. 12. Concentration of both Hoechst 33342 and PY should be maintained in all washing and staining solutions during immunophenotyping staining to prevent efflux of the dyes.
Hoechst concentration
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NettetHoechst 33342 Ready Flow Reagent is a cell-permeable, blue fluorescent DNA stain for cell cycle analysis. Add 2 drops from the dropper bottle directly into 1 x 10 6 cells, incubate, and analyze. Hoechst 33342 reagent binds preferentially to adenine-thymine regions of double-stranded DNA. NettetA concentration of 0.1–12 μg/ml is commonly used to stain DNA in bacteria or eukaryote cells. Cells are stained for 1-30 min at room temperature or 37 °C and then washed to remove unbound dye. A green fluorescence of unbound Hoechst dye may be observed …
NettetFind hoechst 33342 and related products for scientific research at MilliporeSigma. US EN. Applications Products Services Support. Advanced Search. Structure Search. Search Within. Products Technical Documents Site Content Papers Genes Chromatograms. Shipping. Ships Today (1) Product Category. dyes (4) Brand. Sigma-Aldrich (6) … NettetHoechst 33342 Intermediate Stock Solution ; Prepare a dilution of Hoechst 33342 in DPBS for a final concentration of 1 mg/ml. Hoechst 33342 Working Solution : Dilute …
NettetIf you have great powers of concentration, that means you're able to focus all your attention on the matter at hand. Concentration can also refer to something that's … NettetdsDNA solution and unknown sample (final concentration of the Hoechst dye will be 100ng/ml). Note: This assay may be scaled to fit the investigator’s needs (i.e. add …
NettetHoechst 33258核酸染色剂是一种常用的核复染剂，与 dsDNA 结合后会发出蓝色荧光。 Hoechst 33258使用方法与用于复染、凋亡和细胞周期研究的 Hoechst 33342 (H-1399) 相似，但细胞通透性较低。 For Research Use Only. Not for use in diagnostic procedures. 规格 颜色 蓝色 激发波长范围 352 nm 染料类型 Hoechst 33258 适用于（应用） 荧光标 …
Nettet7. okt. 2024 · Hoechst 33342 solution (10 mg/mL in distilled water). The solution is diluted at a concentration of 1 mg/mL with distilled water, filter-sterilized and then stored at −20 °C in 1 mL aliquots in the dark. The “working” stock solution is covered with aluminum foil and kept at 4 °C. 7. steve wilkos mondayNettetHoechst 33258 nucleic acid stain is a popular nuclear counterstain that emits blue fluorescence when bound to dsDNA. Its uses are similar to Hoechst 33342 (H-1399) for counterstaining, apoptosis and cell cycle … steve wilkos new episodes 2021NettetHoechst 33258 Staining Dye Solution (ab228550) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, … steve wilkos show daddy i hate you part 1NettetThe concentration of stain can influence comet assay results, as was shown by Olive et al. ... minor groove-binding Hoechst 33342 and DAPI showed similar sensitivities (indicated by the slopes of dose-response curves), as did bromodeoxyuridine incorporated into replicating DNA and detected with FITC-conjugated anti-BrdUrd (Olive et al., 1992). steve wilkos show abuser plans backfiresNettetRecommended conditions for staining cells with Hoechst stains. Cell Type Hoechst Dye Concentration Incubation Conditions Bacteria 0.1 to 12 µg/mL 10 to 30 minutes Live … steve wilkos show facebookNettet13. okt. 2024 · Add Hoechst dye to a final concentration of 1–10 μg/ml (1.6–16.2 μM). Hoechst concentration requires optimization ( see Note 3 ). 3. Mix well and incubate in a water bath pre-adjusted to 37 °C for 45 min ( see Note 4 ) in the dark. Mix every 15 min. 4. Add Pyronin directly to cells at a final concentration of 1–5 μg/ml (3.3–16.5 μM). steve wilkos on mauryNettetVolume of Water needed to reconstitute specific mass of Hoechst 33342 *20 mM solution in water* to given concentration. Note that volume is only for preparing stock … steve wilkos season 8