WebFor intracellular staining, cells were fixed and permeabilized using Biolegend Foxp3 Fix/Perm Buffer set (BioLegend, San Diego, CA, USA) according to the manufacturer’s instructions, followed by the addition of the corresponding antibodies. Stained cells were stored in 1% paraformaldehyde until analysis but not longer than 24 h. WebApr 6, 2024 · Therefore, targeting immunosuppressive subtypes, primarily FOXP3+ Tregs, ... and then fixed and permeabilized using the Cyto-Fast fix-perm buffer set (BioLegend), as per manufacturer’s ...
Foxp3 / Transcription Factor Fix/Perm Diluent (1X)
WebBioLegend's FOXP3 Fix/Perm Buffer (4x) has been specially formulated for intracellular staining of FOXP3 with minimum effect on the surface fluorochrome staining. The FOXP3 Fix/Perm buffer(4X) must be freshly diluted to 1X working solution with PBS (10 mM … Cyto-Fast™ Fix/Perm Buffer Set . Intracellular Staining Permeabilization … WebDilute the fix/perm buffer 1:3 in dilutant. Add 100ml/well and fix 30 minutes at 4° Wash with 1x perm/wash buffer (dilute 10x stock with water). Can leave cells overnight in perm/wash buffer. Make up Ab panel cocktail in perm/wash buffer. Stain in 100ml volume for 20-30 minutes at 4°C covered in foil. regain and build confidence
SAFETY DATA SHEET - BioLegend
WebJan 21, 2024 · product name : Propidium Iodide Solution catalog : 421301 quantity : 2 mL price : 54 USD more info or order : BioLegend product webpage citations: 74 product information Apps. Abbrev. : Flow Cytometry Cat # : 421301 Item : Propidium Iodide Solution Other Names : PI Size : 2 mL Price (USD) : 54 USD Conjugate/Tag/Label : … WebJan 21, 2024 · product name : Intracellular Staining Perm Wash Buffer (10X) catalog : 421002 quantity : 100 mL price : 94 USD more info or order : BioLegend product webpage citations: 61 product information Apps. Abbrev. : ICC, Intracellular Staining for Flow Cytometry, IHC Cat # : 421002 Item : Intracellular Staining Perm Wash Buffer (10X) … WebBD Phosflow Perm Buffer IV BDB560746 Strong detergent Solution Useful for some BD Phosflow applications Alternative Permeabilization Buffers Name Cat. No. Contents Comments BD Pharmingen™ Transcription Factor Buffer Set BDB562574 BD Cytofix/Cytoperm fixation/permeabilization buffer, Transcription Factor Fix/Perm Buffer, regain arras