WebJan 17, 2012 · Fluorescence intensities of the resuspended cells were quantified via FACScan flow cytometer (Epics Altra, Beckman Coulter, Miami, USA) using excitation and emission wavelengths of 485 and 535 nm, respectively. Additionally, these experiments were performed with the presence of the antioxidant Cys at concentrations of 80 mM. … WebSummary: An agile epic is a body of work that can be broken down into specific tasks (called user stories) based on the needs/requests of customers or end-users. Epics are an important practice for agile and DevOps teams. When adopting agile and DevOps, an epic serves to manage tasks. It's a defined body of work that is segmented into specific ...
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WebNov 15, 2005 · Isolation of GYPA +, CD71 + cells and GYPA + and CD71-cells was performed by cell sorting (EPICS Altra, Beckman Coulter, Miami, FL) and confirmed to be more than 91% pure. Genomic DNA was generated from the isolated cells and submitted for chimerism analysis. WebJan 14, 2010 · Just before FCM analysis, propidium iodide (Molecular Probes) solution was added at a final concentration of 2 μg/mL to identify nonviable cells. FCM analysis was performed on a dual laser (488 nm and ultraviolet) flow cytometer (EPICS ALTRA; Beckman Coulter), as previously described. 11 how to do a bench press properly
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WebAug 1, 2005 · Cells were then fixed in ethanol, stained in propidium iodide and analyzed using a Beckman-Coulter EPICS ALTRA flow cytometer. Results A recent study regarding the function and regulation of p53 in renal carcinoma cells suggested that p53 function is compromised by a novel dominant mechanism ( 6 ). WebEPICS® ALTRA™ with HyPerSort™ Cell Sorting. Sponsored Site. Send an Inquiry to this supplier. Send an Inquiry to this supplier * From (E-mail) To: Chongyeol Lee Anse Co., Ltd. * Subject * Message: Use English only Max. 2000 characters. (Min. 20) WebJan 5, 2016 · Cells were firstly stained with SPRD-anti-CD4, fixed, and permeabilized, and then stained for intracellular IL-17 with PE-anti-IL-17A mAb. Samples were acquired on Epics Altra, and data were analyzed with EXPO32 Analysis software. Representative histograms of IL-17+ cells gated for CD4 were obtained from a flow cytometric analysis. … how to do a bend on inventor