Bondtm epitope retrieval solution 2 ph 9.0
WebThis antibody detects both long and short forms of STAT2. Tested applications Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P more details Species reactivity Reacts with: Mouse, Rat, Human Immunogen Synthetic peptide within Human STAT2 (N terminal). The exact sequence is proprietary. Positive control
Bondtm epitope retrieval solution 2 ph 9.0
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WebmAb clone 4B11, product no. PA0183, Leica, Bond-max/Bond-III: Protocols with optimal results were based on HIER using BERS2 pH 9 (efficient heating time 20 min. at 95 … WebSearch for: BOND Epitope Retrieval Solution 2 – AR9640
WebPerform heat-mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. ICC/IF: 1/1000. IP: 1/30. Notes; WB 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 31 kDa). Human and rat species are recommended based on IHC result. This antibody is unsuitable to be used in western … WebGeneral notes This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free production For more information see here.
WebEDTA based antigen retrieval (Leica Biosystems BOND ® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. WebEDTA based antigen retrieval (Leica Biosystems BOND ® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
WebThey are provided as a sampler panel to allow you to easily evaluate each antibody. For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody. Panel contains: - Rabbit monoclonal [73-10] to PD-L1 (20 μL) ab228415 - Rabbit monoclonal [EPR4877 (2)] to PD1 (20 μL) ab137132
WebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - … handbrake windows 10 italianoWebWB: THP1 cell lysate treated with TPA, and TF1 cell lysate; Rat spleen lysate IHC-P: Human tonsil and spleen tissues; Rat cerebrum and bone marrow tissue; Mouse lung … buses to bridport from beaminsterWebEDTA based antigen retrieval (Leica Biosystems BOND ® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous … buses to broadland business parkWebDescription Rabbit monoclonal [E90] to EIF2S1 (phospho S51) Host species Rabbit Specificity Anti-EIF2S1 (phospho S51) antibody [E90] (ab32157) only detects eIF-2a phosphorylated on Serine 51. Tested applications … handbrake windows 10 issuesWebHeat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. Flow Cytometry (Intracellular) - Anti-TUBA1B antibody [EPR1333] … buses to brierley hillWebThe sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab92471 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB ( ab209101 ). handbrake windows 10 gratisProduct ID: epitope-retrieval-solution-2 BOND ancillaries have been designed for Leica Biosystems fully-automated BOND systems: BOND-MAX and BOND-III systems. Used in the development and validation of BOND ready-to-use primary antibodies and ISH probes - choose Leica Biosystems reagents for the assurance of a quality result in your laboratory. buses to bromley south